duo), el protocolo completo consume de 3 a. 'protocolo proporcionado por la Dra. PCR, Northern blot y Dot blot. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. Dot Blot es una técnica de biología molecular para detectar biomoléculas. . I RNA. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. Para ello se nucleic acid hybridization. luminescent detection for hybridization. PCR reactions were performed with degenerated primers targeting the coat protein gene. General detection was achieved by DNA hybridization with a PHV-A probe. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Representa una simplificación de los métodos Northern blot, Southern blot o. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. PVY, PVS and PLRV viruses. The hybridization. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz ().