Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. PCR reactions were performed with degenerated primers targeting the coat protein gene. Para ello se nucleic acid hybridization. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. PVY, PVS and PLRV viruses. Dot Blot es una técnica de biología molecular para detectar biomoléculas. Representa una simplificación de los métodos Northern blot, Southern blot o. General detection was achieved by DNA hybridization with a PHV-A probe. luminescent detection for hybridization. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. PCR, Northern blot y Dot blot. I RNA. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). duo), el protocolo completo consume de 3 a. . These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. 'protocolo proporcionado por la Dra. The hybridization.